Features and Benefits

Compatible with high-throughput handling systems.

General description

Succinyl-CoA synthetase (SCS; succinyl-CoA ligase; succinate thiokinase; EC 6.2.1.5), also known as succinyl-CoA synthetase ligase/succinate thiokinase, is a mitochondrial enzyme. The enzyme catalyzes a substrate-level phosphorylation of GDP or ADP and thus, plays a vital role in Krebs cycle. In addition, it also catalyzes the reverse reaction to supply succinyl-CoA for heme synthesis and ketone body activation. Deficiency in SCS activity is associated with encephalomyopathy and depletion of mitochondrial DNA.Aberrations in the gene expression leads to the development of diabetes and neurodegenerative diseases.

Principle

The Succinyl-CoA Synthetase Activity Colorimetric Assay Kit is a simple and high throughput adaptable assay for measuring SCS activity in a variety of tissues, cells, and isolated mitochondria. SCS activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of succinyl-CoA synthetase is the amount of enzyme that generates 1.0 µmole of NADH per minute at pH 7.4 at 25 °C.

Suitability

Suitable for the detection of Succinly-CoA synthetase in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.